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Virtual Microscopy for Parasitology

The aim of this project is to adapt virtual microscopy for parasitology specimens. The project was motivated by difficulties in education and quality assurance in a SIDA/Sarec sponsored co-operative effort on control of intestinal parasites in Nicaragua.

Such difficulties are however not restricted to developing countries. Quality assurance, e.g. for stool samples, is problematic basically because identical specimens cannot be distributed to participating parasitology laboratories.

Virtual microscopy addresses major problems, such as specimen handling, availability, reproducibility, distribution and interpretation. A journal article has been published in the
PLoS Neglected Tropical Diseases Journal.
Schistosoma mansoni
Schistosoma mansoni eggs in tissues of infected mouse.
Sample 1
Sample 2
Leishmania paramensis
Sample 1
Blood smears containing malaria parasites
Sample 1
Sample 2
Schistosoma mansoni immunofluorescence
Frozen sections of S. mansoni to show reactivity of antibodies encountered in serum from recently infected individual directed against gut associated antigens (anti-GAA) in Sample 1 and in chronic schisrosomiasis (anti-SA in Sample 2). Immunofluorescence (IF) microscopy as described in reference. (PubMed)
Sample 1
Sample 2
Sample slides with multiple layers
Sample slides with multiple layers, allowing focusing in the browser. You can control the focus depth by dragging the slider in the right margin with the mouse.
Stool sample 1,
8 layers
Stool sample 2,
10 layers
Swedish Institute for Infectious Disease Control (SMI), S-171 82 Solna, Sweden
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet
Department of Microbiology, National University of Leon (UNAN), Leon, Nicaragua
Biomedical Informatics Group, University of Helsinki, HUCH Clinical Research Institute, Helsinki, Finland
Division of Pathology, HUSLAB, Helsinki University Central Hospital FIN-00290 Helsinki, Finland
Institute of Medical Technology, University of Tampere, 33014 Tampere, Finland.
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